NKX2.2 and KLF4 cooperate to regulate α cell identity

Brooks EP, Casey MR, Wells KL, Liu TY, Van Orman M, Sussel L. NKX2.2 and KLF4 cooperate to regulate α-cell identity. Genes Dev. 2024 Dec 20. doi: 10.1101/gad.352193.124. Epub ahead of print. PMID: 39797760.

Islet-antigen reactive B cells display a unique phenotype and BCR repertoire in autoantibody positive and type 1 diabetes patient

Nicholas CA, Tensun FA, Evans SA, Toole KP, Broncucia H, Hesselberth JR, Gottlieb PA, Wells KL, Smith MJ. Islet-antigen reactive B cells display a unique phenotype and BCR repertoire in autoantibody positive and recent-onset type 1 diabetes patients. bioRxiv [Preprint]. 2024 Jun 25:2024.06.20.599914. doi: 10.1101/2024.06.20.599914. PMID: 38979376; PMCID: PMC11230262.

CFTR Repressed a PDX1 Axis to Govern Pancreatic Ductal Cell Fate

Rotti PG, Yi Y, Gasser G, Yuan F, Sun X, Apak-Evans I, Wu P, Liu G, Choi S, Reeves R, Scioneaux AE, Zhang Y, Winter M, Liang B, Cunicelli N, Uc A, Norris AW, Sussel L, Wells KL, Engelhardt JF. CFTR represses a PDX1 axis to govern pancreatic ductal cell fate. iScience. 2024 Nov 15;27(12):111393. doi: 10.1016/j.isci.2024.111393. PMID: 39687022; PMCID: PMC11647141.

RNA binding protein PCBP2 regulates β cell function and adaptation to glucose

Haemmerle MW, Scota AV, Khosravifar M, Varney MJ, Sen S, Good AL, Yang X, Wells KL, Sussel L, Rozo AV, Doliba NM, Ghanem LR, Stoffers DA. RNA-binding protein PCBP2 regulates pancreatic β cell function and adaptation to glucose. J Clin Invest. 2024 Jun 17;134(12):e172436. doi: 10.1172/JCI172436. PMID: 38950317; PMCID: PMC11178539.

Uterine injury during diestrus leads to placental and embryonic defects in future pregnancies in mice

Zhang ET, Wells KL, Bergman AJ, Ryan EE, Steinmetz LM, Baker JC. Uterine injury during diestrus leads to placental and embryonic defects in future pregnancies in mice†. Biol Reprod. 2024 Apr 11;110(4):819-833. doi: 10.1093/biolre/ioae001. PMID: 38206869; PMCID: PMC11017118.

Modulation of insulin secretion by RBFOX2-mediated alternative splicing

Moss ND, Wells KL, Theis A, Kim YK, Spigelman AF, Liu X, MacDonald PE, Sussel L. Modulation of insulin secretion by RBFOX2-mediated alternative splicing. Nat Commun. 2023 Nov 25;14(1):7732. doi: 10.1038/s41467-023-43605-4. PMID: 38007492; PMCID: PMC10676425.

Transgenic Ferret Models Define Pulmonary Ionocyte Diversity and Function

Yuan F, Gasser GN, Lemire E, Montoro DT, Jagadeesh K, Zhang Y, Duan Y, Ievlev V, Wells KL, Rotti PG, Shahin W, Winter M, Rosen BH, Evans I, Cai Q, Yu M, Walsh SA, Acevedo MR, Pandya DN, Akurathi V, Dick DW, Wadas TJ, Joo NS, Wine JJ, Birket S, Fernandez CM, Leung HM, Tearney GJ, Verkman AS, Haggie PM, Scott K, Bartels D, Meyerholz DK, Rowe SM, Liu X, Yan Z, Haber AL, Sun X, Engelhardt JF. Transgenic ferret models define pulmonary ionocyte diversity and function. Nature. 2023 Sep;621(7980):857-867. doi: 10.1038/s41586-023-06549-9. Epub 2023 Sep 20. PMID: 37730992; PMCID: PMC10533402.

Major β cell-specific functions of NKX2.2 are mediated via the NK2-specific domain

Abarinov V, Levine JA, Churchill AJ, Hopwood B, Deiter CS, Guney MA, Wells KL, Schrunk JM, Guo Y, Hammelman J, Gifford DK, Magnuson MA, Wichterle H, Sussel L. Major β cell-specific functions of NKX2.2 are mediated via the NK2-specific domain. Genes Dev. 2023 Jun 1;37(11-12):490-504. doi: 10.1101/gad.350569.123. Epub 2023 Jun 26. PMID: 37364986; PMCID: PMC10393193.

Identification of an anergic BND cell-derived activated B cell population (BND2) in young-onset type 1 diabetes patients

Stensland ZC, Magera CA, Broncucia H, Gomez BD, Rios-Guzman NM, Wells KL, Nicholas CA, Rihanek M, Hunter MJ, Toole KP, Gottlieb PA, Smith MJ. Identification of an anergic BND cell-derived activated B cell population (BND2) in young-onset type 1 diabetes patients. J Exp Med. 2023 Aug 7;220(8):e20221604. doi: 10.1084/jem.20221604. Epub 2023 May 15. PMID: 37184563; PMCID: PMC10192302.

Zinc Transporter 8 Haploinsufficiency Protects Against Beta Cell Dysfunction in Type 1 Diabetes by Increasing Mitochondrial Respiration

Kim YK, Walters JA, Moss ND, Wells KL, Sheridan R, Miranda JG, Benninger RKP, Pyle LL, O'Brien RM, Sussel L, Davidson HW. Zinc transporter 8 haploinsufficiency protects against beta cell dysfunction in type 1 diabetes by increasing mitochondrial respiration. Mol Metab. 2022 Dec;66:101632. doi: 10.1016/j.molmet.2022.101632. Epub 2022 Nov 5. PMID: 36347424; PMCID: PMC9672421.

Small cell carcinoma of the ovary hypercalcemic type (SCCOHT): A review and novel case with dual germline SMARCA4 and BRCA2 mutations

Sanders BE, Wolsky R, Doughty ES, Wells KL, Ghosh D, Ku L, Pressey JG, Bitler BB, Brubaker LW. Small cell carcinoma of the ovary hypercalcemic type (SCCOHT): A review and novel case with dual germline SMARCA4 and BRCA2 mutations. Gynecol Oncol Rep. 2022 Oct 6;44:101077. doi: 10.1016/j.gore.2022.101077. PMID: 36249907; PMCID: PMC9554814.

Dynamic changes in β-cell [Ca2+] regulates NFAT activation, gene transcription and islet gap junction communication

Miranda JG, Schleicher WE, Wells KL, Ramirez DG, Landgrave SP, Benninger RKP. Dynamic changes in β-cell [Ca2+] regulate NFAT activation, gene transcription, and islet gap junction communication. Mol Metab. 2022 Mar;57:101430. doi: 10.1016/j.molmet.2021.101430. Epub 2021 Dec 31. PMID: 34979329; PMCID: PMC8804269.

Integrated single-cell transcriptomics and epigenomics reveals strong germinal center-associated etiology of autoimmune risk loci.

King HW, Wells KL, Shipony Z, Kathiria AS, Wagar LE, Lareau C, Orban N, Capasso R, Davis MM, Steinmetz LM, James LK, Greenleaf WJ. Integrated single-cell transcriptomics and epigenomics reveals strong germinal center-associated etiology of autoimmune risk loci. Sci Immunol. 2021 Oct 15;6(64):eabh3768. doi: 10.1126/sciimmunol.abh3768. Epub 2021 Oct 8. PMID: 34623901; PMCID: PMC8859880.

Combined transient ablation and single cell RNA sequencing reveals the development of medullary thymic epithelial cells

Wells KL, Miller CN, Gschwind AR, Wei W, Phipps JD, Anderson MS, Steinmetz LM. Combined transient ablation and single-cell RNA-sequencing reveals the development of medullary thymic epithelial cells. Elife. 2020 Nov 23;9:e60188. doi: 10.7554/eLife.60188. PMID: 33226342; PMCID: PMC7771965.

Thymic tuft cells promote an IL-4-enriched medulla and shape thymocyte development.

Miller CN, Proekt I, von Moltke J, Wells KL, Rajpurkar AR, Wang H, Rattay K, Khan IS, Metzger TC, Pollack JL, Fries AC, Lwin WW, Wigton EJ, Parent AV, Kyewski B, Erle DJ, Hogquist KA, Steinmetz LM, Locksley RM, Anderson MS. Thymic tuft cells promote an IL-4-enriched medulla and shape thymocyte development. Nature. 2018 Jul;559(7715):627-631. doi: 10.1038/s41586-018-0345-2. Epub 2018 Jul 18. PMID: 30022164; PMCID: PMC6062473.

A splice acceptor mutation in C. elegans daf-19/Rfx disrupts functional specialization of male-specific cilliated neurons but does not affect ciliogenesis

Wells, KL, Rowenki, M, Killian, DJ. A splice acceptor mutation in C. elegans daf-19/Rfx disrupts functional specialization of male-specific cilliated neurons but does not affect ciliogenesis. Gene. 2015 April; 559: 196-202.

Conserved RNA-Binding Proteins Required for Dendrite Morphogenesis in Caenorhabditis elegans Sensory Neurons

Antonacci S, Forand D, Wolf M, Tyus C, Barney J, Kellogg L, Simon MA, Kerr G, Wells KL, Younes S, Mortimer NT, Olesnicky EC, Killian DJ. Conserved RNA-binding proteins required for dendrite morphogenesis in Caenorhabditis elegans sensory neurons. G3 (Bethesda). 2015 Feb 10;5(4):639-53. doi: 10.1534/g3.115.017327. PMID: 25673135; PMCID: PMC4390579.